Journal:

Article Title: Chemokine and chemokine receptor expression during colony stimulating factor-1-induced osteoclast differentiation in the toothless osteopetrotic rat: a key role for CCL9 (MIP-1?) in osteoclastogenesis in vivo and in vitro

doi: 10.1182/blood-2005-08-3365

Figure Lengend Snippet: Relative gene expression for osteoclast-associated genes, chemokines, and chemokine receptors

Article Snippet: For studies of in vivo inhibition of osteoclast differentiation by anti-CCL9, tl / tl rats were injected at 20 days after birth with 50 μg affinity-purified rabbit anti-murine CCL9 (MIP-1γ) antibody (Peprotech, Rocky Hill, NJ) in 100 μL PBS, and again on days 21 and 23, with 50 μg anti-CCL9 plus 10 6 U CSF-1 in 100 μL PBS.

Techniques: Expressing, Microarray

Journal:

Article Title: Chemokine and chemokine receptor expression during colony stimulating factor-1-induced osteoclast differentiation in the toothless osteopetrotic rat: a key role for CCL9 (MIP-1?) in osteoclastogenesis in vivo and in vitro

doi: 10.1182/blood-2005-08-3365

Figure Lengend Snippet: CCL-9 and CCR1 mRNA expression in long bones determined by microarray and real-time PCR. tl rats were treated for the days indicated and total bone RNA was analyzed. CCL9 and CCR1 mRNA were highest after 2 days of treatment, after which the levels subsided. Means + 1 SD are shown. Compared with untreated animals (day 0), all time points were significantly elevated. (A-B) *Difference from day 0, P < .01. (C) *Difference from day 0, P < .005; **P < .02.

Article Snippet: For studies of in vivo inhibition of osteoclast differentiation by anti-CCL9, tl / tl rats were injected at 20 days after birth with 50 μg affinity-purified rabbit anti-murine CCL9 (MIP-1γ) antibody (Peprotech, Rocky Hill, NJ) in 100 μL PBS, and again on days 21 and 23, with 50 μg anti-CCL9 plus 10 6 U CSF-1 in 100 μL PBS.

Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction

Journal:

Article Title: Chemokine and chemokine receptor expression during colony stimulating factor-1-induced osteoclast differentiation in the toothless osteopetrotic rat: a key role for CCL9 (MIP-1?) in osteoclastogenesis in vivo and in vitro

doi: 10.1182/blood-2005-08-3365

Figure Lengend Snippet: Inhibition of CCL9 blocks osteoclast differentiation in vitro. Rat BMCs were induced to differentiate into osteoclasts using CSF-1 and RANKL. Anti-CCL9 antibody blocked the formation of TRAP-positive, multinucleated cells. Means + 1 SD are shown. For both antibody concentrations tested, the decrease was highly significant. *P < .001.

Article Snippet: For studies of in vivo inhibition of osteoclast differentiation by anti-CCL9, tl / tl rats were injected at 20 days after birth with 50 μg affinity-purified rabbit anti-murine CCL9 (MIP-1γ) antibody (Peprotech, Rocky Hill, NJ) in 100 μL PBS, and again on days 21 and 23, with 50 μg anti-CCL9 plus 10 6 U CSF-1 in 100 μL PBS.

Techniques: Inhibition, In Vitro

Journal:

Article Title: Chemokine and chemokine receptor expression during colony stimulating factor-1-induced osteoclast differentiation in the toothless osteopetrotic rat: a key role for CCL9 (MIP-1?) in osteoclastogenesis in vivo and in vitro

doi: 10.1182/blood-2005-08-3365

Figure Lengend Snippet: Injections of anti-CCL9 suppress osteoclast differentiation in vivo. tl/tl rats were injected either with CSF-1 alone or with anti-CCL9 antibody and CSF-1, and TRAP+ osteoclasts were counted on multiple sections of the proximal femur from 3 animals for each condition after 4 days of injections. Anti-CCL9 significantly reduced the osteoclastogenic response to CSF-1 injections. Means + 1 SD are shown. In both areas, differences were highly significant (*P < .001).

Article Snippet: For studies of in vivo inhibition of osteoclast differentiation by anti-CCL9, tl / tl rats were injected at 20 days after birth with 50 μg affinity-purified rabbit anti-murine CCL9 (MIP-1γ) antibody (Peprotech, Rocky Hill, NJ) in 100 μL PBS, and again on days 21 and 23, with 50 μg anti-CCL9 plus 10 6 U CSF-1 in 100 μL PBS.

Techniques: In Vivo, Injection

Journal: Nutrients

Article Title: Anti-Stress, Glial- and Neuro-Differentiation Potential of Resveratrol: Characterization by Cellular, Biochemical and Imaging Assays

doi: 10.3390/nu12030671

Figure Lengend Snippet: Molecular changes in control and resveratrol-treated stressed cells. ( A ). Western blot showing induction of DNA damage signaling and growth arrest with various chemical stresses listed in ( B ). Resveratrol-treated cells showed considerable reversal of the molecular changes. ( C ). Immunostaining showing induction of DNA damage (γH2AX) and senescence (HP1γ) in stressed cells. Resveratrol-treated cells showed considerable recovery. The decrease in mortalin in cells stressed with some stresses was recovered with resveratrol treatment.

Article Snippet: Primary antibodies against Luciferase (AbCam, ab16466), γH2AX (Cell Signaling, 9718S), pCHK1 (Cell Signaling, 2344S), pCHK2 (Cell Signaling, 2197P), HP1γ (Merck Millipore, 05–690), Mortalin [ ], β-actin (AbCam, ab49900), GFAP (Sigma-Aldrich, G9269), Nestin (Santa Cruz, SC-23927), MAP2 (Sigma-Aldrich, M3696), PSD-95 (Santa Cruz, SC-32290), Neuropsin (Santa Cruz, SC-134600), GAP43 (Santa Cruz, SC-33705), NCAM (Santa Cruz, SC-10735), Survivin (Santa Cruz, SC-10811), Vimentin (Santa Cruz, SC-6260), NeuN (AbCam, ab177487), TCF4 (Santa Cruz, SC-166699), SMAD2/3 (Cell Signaling, 8685S), pSMAD2/3 (Cell Signaling, 8828), hnRNP-K (Cell Signaling, 4675S), E-cadherin (Cell Signaling, 5296S), and MMP2 (Santa Cruz, SC-13594) proteins were used for immunostaining or blotting as indicated.

Techniques: Western Blot, Immunostaining